Analytical Sciences

Abstract − Analytical Sciences, 29(1), 157 (2013).

An Application of Polymer-Enhanced Capillary Transient Isotachophoresis with an Emissive Boronic Acid Functionalized Squarylium Dye as an On-Capillary Labeling Agent for Gram-positive Bacteria
Shingo SAITO,*,** Takeshi MAEDA,*** Hiroyuki NAKAZUMI,*** and Christa L. COLYER**
*Graduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Sakura, Saitama 338-8570, Japan
**Department of Chemistry, Wake Forest University, Winston-Salem, NC 27109, USA
***Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuen, Naka, Sakai, Osaka 599-8531, Japan
In this paper, the characterization and application of the “PectI” (polymer-enhanced capillary transient isotachophoresis) technique for the separation and detection of same genus, gram-positive bacteria, Bacillus globigii (Bg) and Bacillus subtilis, is demonstrated by employing a boronic acid-functionalized squarylium dye (SQ-BA) as an on-capillary labeling agent, including the quantitative performance and applicability to crude samples. The effect of borate in the separation buffer was also investigated, which revealed that borate strongly affects the separation behavior of bacteria.