Analytical Sciences

Chromatographic Separation Studies of Penicillins, Cephalosporins and Carbapenems on Transition-Metal Silicate Modified Silica Layers

Dhruv K. SINGH and Gunjan MAHESHWARI

Analytical Research Laboratory, Department of Chemistry, Harcourt Butler Technological Institute, Kanpur-208002, India

The chromatographic behavior of penicillins, cephalosporins and carbapenems has been studied on the thin layers of transition-metal ion (viz. Ni²⁺/Zn²⁺/Cu²⁺/Co²⁺) silicate modified silica. Transition-metal silicate (3.92%) and silica (96.08%) were found to be optimum and resulted in spherical-compact spots and improved resolution of the analytes. The effect of various mobile phases was also investigated. The chromatograms were visualized as yellow spots by placing in an I₂-chamber. The method has been found to be reproducible and convenient for routine analysis.

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