Analytical Sciences

Abstract − Analytical Sciences, 28(3), 197 (2012).

Micro Total Bioassay System for Oral Drugs: Evaluation of Gastrointestinal Degradation, Intestinal Absorption, Hepatic Metabolism, and Bioactivity
Yuki IMURA,* Etsuro YOSHIMURA,* and Kiichi SATO*,**
*Department of Applied Biological Chemistry, School of Agricultural and Life Science, The University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo 113-8657, Japan
**Department of Chemistry and Chemical Biology, School of Engineering, Gunma University, 1-5-1 Tenjin, Kiryu, Gunma 376-8515, Japan
A micro total bioassay system that mimics physiological processes was developed as a means of evaluating orally administered drugs. A new feature accounting for gastrointestinal digestion was added to the previous system, which consists of microintestine, microliver, and target components. The artificial micro-gastrointestinal tract employs synthetic digestive juices. The system could correctly assay the overall digestive properties of ingested anticancer agents, i.e., the stability during digestive processes, as well as intestinal absorption, hepatic metabolism, and bioactivity toward target cells.