Analytical Sciences


Abstract − Analytical Sciences, 28(2), 187 (2012).

Development of an Electrochemical Cholesterol Sensor System for Food Analysis
Tsutomu NAGAOKA,* Shiho TOKONAMI,** Hiroshi SHIIGI,* Hiroaki MATSUMOTO,* Yasuhiro TAKAGI,*** and Yasunori TAKAHASHI***
*Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-2 Gakuen-cho, Naka, Sakai 599-8570, Japan
**Nanoscience and Nanotechnology Research Center, Research Organization for the 21th Century, Osaka Prefecture University, 1-2 Gakuen-cho, Naka, Sakai 599-8570, Japan
***JSK Co. Ltd., 1-5-1 Higashi-amakawa, Takatsuki, Osaka 560-0012, Japan
In this article, we report on a food-cholesterol monitoring sensor based on a non-enzymatic approach. Amorphous and single-crystal gold electrodes were modified with an alkanethiol self-assembled monolayer to quantify it by voltammetry. We first discuss the basic characteristics of these sensors and provide more information about the instrument developed by JSK Co. This instrument is a battery-operated handheld voltammetric analyzer, which mounts a sensor chip to monitor cholesterol contents in food samples. The sensor showed excellent linearity with the cholesterol concentration; egg-yolk samples were analyzed to give an excellent agreement between the values obtained by the sensor (1.4 mM) and chromatography (1.1 mM).