Analytical Sciences

Development of an Electrochemical Cholesterol Sensor System for Food Analysis

Tsutomu NAGAOKA,* Shiho TOKONAMI,** Hiroshi SHIIGI,* Hiroaki MATSUMOTO,* Yasuhiro TAKAGI,*** and Yasunori TAKAHASHI***

*Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-2 Gakuen-cho, Naka, Sakai 599-8570, Japan
**Nanoscience and Nanotechnology Research Center, Research Organization for the 21th Century, Osaka Prefecture University, 1-2 Gakuen-cho, Naka, Sakai 599-8570, Japan
***JSK Co. Ltd., 1-5-1 Higashi-amakawa, Takatsuki, Osaka 560-0012, Japan

In this article, we report on a food-cholesterol monitoring sensor based on a non-enzymatic approach. Amorphous and single-crystal gold electrodes were modified with an alkanethiol self-assembled monolayer to quantify it by voltammetry. We first discuss the basic characteristics of these sensors and provide more information about the instrument developed by JSK Co. This instrument is a battery-operated handheld voltammetric analyzer, which mounts a sensor chip to monitor cholesterol contents in food samples. The sensor showed excellent linearity with the cholesterol concentration; egg-yolk samples were analyzed to give an excellent agreement between the values obtained by the sensor (1.4 mM) and chromatography (1.1 mM).

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