Analytical Sciences

Abstract − Analytical Sciences, 28(10), 1013 (2012).

Immobilization of Bacterial Luciferase into Poly(N-isopropylacrylamide) Film for Electrochemical Control of a Bioluminescence Reaction
Yoji KAWANAMI,* Shinya YAMASAKI,** Shuto YAMADA,*** and Kô TAKEHARA*
*Department of Chemistry, Faculty of Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi, Fukuoka 812-8581, Japan
**Advanced Science Research Center, Japan Atomic Energy Agency, Tokai, Ibaraki 319-1195, Japan
***Faculty of Arts and Science, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan
A poly(N-isopropylacrylamide) film was modified on an indium-tin oxide electrode in order to immobilize bacterial luciferase (BL) on the electrode surface. By using the modified electrode, flavin mononucleotide (FMN) was electrochemically reduced to FMNH2, which is one of the substrates of the BL luminescence reaction, to control the bioluminescence reaction by BL. The BL reaction in the modified film could be promoted and controlled by the electrochemical generation of FMNH2. This BL luminescence system was evaluated as a model system for the inhibitory assay of hydrophobic molecules on protein functions.