Analytical Sciences

Abstract − Analytical Sciences, 28(1), 73 (2012).

Naked-eye Detection of Amyloid Aggregates Using Gold Nanoparticles Modified with Amyloid Beta Antibody
Masafumi SAKONO,*,** Tamotsu ZAKO,** and Mizuo MAEDA**
*PRESTO, Japan Science and Technology Agency, 3-5 Sanbancho, Chiyoda, Tokyo 102–0075, Japan
**Bioengineering Laboratory, RIKEN Institute, 2-1 Hirosawa, Wako, Saitama 351–0198, Japan
We developed a rapid method for estimating the amyloid beta (Aβ)-conformation state related with Alzheimer’s disease. We prepared gold nanoparticle (AuNP)-Aβ antibody conjugates treated with bovine serum albumin to stabilize their dispersibility in a buffer. The prepared AuNPs were precipitated in the presence of Aβ aggregates, such as oligomers and fibrils. Aβ monomers did not precipitate AuNPs. The formation of AuNP precipitates by Aβ aggregates could be confirmed by the naked eye within 1 h.