Analytical Sciences

Abstract − Analytical Sciences, 27(8), 811 (2011).

High-Temperature Separations on a Polymer-Coated Fibrous Stationary Phase in Microcolumn Liquid Chromatography
Kenichi NAKANE,* Shingoro SHIRAI,* Yoshihiro SAITO,* Yusuke MORIWAKE,* Ikuo UETA,** Mitsuru INOUE,*,*** and Kiyokatsu JINNO*
*Department of Environmental and Life Sciences, Toyohashi University of Technology, Toyohashi 441-8580, Japan
**Department of Applied Chemistry, University of Yamanashi, 4-3-11 Takeda, Kofu 400-8511, Japan
***Okazaki Technical Senior High School, Okazaki 444-8555, Japan
Novel polymer-coated fiber-packed microcolumns in liquid chromatography (LC) have been developed. Typical polymeric materials, such as polydimethylsiloxane and polyethyleneglycol, which are conventional stationary phases of capillary columns in gas chromatography (GC), have been employed as coating materials onto the surface of fine filaments. Packed longitudinally with a bundle of polymer-coated filaments into a stainless-steel capillary of 0.8 mm i.d., 150 mm length, several types of polymer-coated fiber-packed columns were prepared, and the retention behavior of aromatic compounds on these columns has been studied. A good linear relationship was obtained for van’t Hoff plots over the temperature range between 0 and 200°C, clearly indicating an excellent heat-resistant property of these polymer-coated fibrous stationary phases. Taking advantage of the heat-resistant feature of the fibrous stationary phases, the separation of several test mixtures with temperature-programmed elution was studied, where a solvent gradient program was additionally introduced if needed. Separation was also carried out with pure water as the mobile phase using an appropriate temperature program.