Analytical Sciences

Abstract − Analytical Sciences, 27(4), 363 (2011).

Direct Detection of Uric Acid in Body Fluid Samples by Using Boron Doped Graphite Nano Particles as the Working Electrode
Jianjun HE, Bunshi FUGETSU, and Shunitz TANAKA
Laboratory of Environmental Remediation, Graduate School of Environmental Science, Hokkaido University, Sapporo 060-0810, Japan
Boron doped graphite nano particles were used as functioning elements for creation of electrodes for the detection of uric acid in biological samples. The electrode obtained in this manner was capable of oxidizing ascorbic acid at lower potentials. This provided a desirable solution to the interfering problem encountered in the detection of uric acid in biological samples caused by ascorbic acid. The detectable concentrations for uric acid ranged from 5.0 to 130 μM. The applicability of the electrode was experimentally demonstrated by the successful direct detection of uric acid in real urine samples.