Analytical Sciences

An Abnormal Temperature Dependence of Alkylpyrazines’ Retention in Reversed-Phase Liquid Chromatography

Kentaro YOGO,*,** Chiharu TAKEMURA,* Yoshihiro SAITO,* and Kiyokatsu JINNO*

*Department of Environmental and Life Sciences, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempakucho, Toyohashi 441-8580, Japan
**Morinaga & Co., Ltd., 2-2-1 Shimosueyoshi, Tsurumi, Yokohama 230-8504, Japan

Retention behaviors of pyrazine and alkylpyrazines on various stationary phases in reversed-phase liquid chromatography were examined. An abnormal temperature effect on the retention of alkylpyrazines with a mobile phase consisting of acetonitrile and water was observed when changing the column temperature. On the other hand, no similar trend was found with a methanol–water mobile phase. For all the columns investigated in this work, the above tendency to the temperature-dependence was consistently observed, suggesting that the abnormal temperature effect on the retention of alkylpyrazines could be mainly induced by an acetonitrile-based mobile phase.

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