Analytical Sciences

Abstract − Analytical Sciences, 27(11), 1065 (2011).

Sensitive Electrochemical Detection of the Hydroxyl Radical Using Enzyme-catalyzed Redox Cycling
Hirosuke TATSUMI* and Naoya OSAKU**
*International Young Researchers Empowerment Center, Shinshu University, Matsumoto, Nagano 390-8621, Japan
**Department of Chemistry, Faculty of Science, Shinshu University, Matsumoto, Nagano 390-8621, Japan
Enzyme-catalyzed signal amplification was introduced to the electrochemical detection of the OH radical. In the presence of phenol as a trapping agent, glucose as a substrate, and pyrroloquinoline quinone-containing glucose dehydrogenase (PQQ-GDH) as a catalyst, the current signal for the trapping adducts (catechol and hydroquinone) produced by the hydroxylation of phenol could be amplified and detected sensitively. The limit of detection (S/N = 3) for catechol was 8 nM. The trapping efficiency of phenol was also estimated.