Analytical Sciences

Abstract − Analytical Sciences, 27(1), 101 (2011).

In-Gel Staining of Proteins in Native Poly Acryl Amide Gel Electrophoresis Using Tetrakis(4-sulfonato phenyl)porphyrin
Kalivarathan DIVAKAR, Vijayan SUJATHA, Sridhar BARATH, Krishnamurthy SRINATH, and Pennathur GAUTAM
Centre for Biotechnology, Anna University Chennai, Chennai-600 025, India
Protein identification in polyacrylamide gel electrophoresis (PAGE) requires post-electrophoretic steps like fixing, staining and destaining of the gel, which are time-consuming and cumbersome. We have developed a method for direct visualization of protein bands in PAGE using tetrakis(4-sulfonato phenyl)porphyrin (TPPS) as a dye without the need for any post electrophoretic steps, where separation and recovery of enzymes become much easier for further analysis. Activity staining was done to prove that the biochemical activity of the enzymes was preserved after electrophoresis.