Analytical Sciences

Abstract − Analytical Sciences, 26(9), 995 (2010).

Mesoporous Platinum Electrodes for Amperometric Determination of Sugars with Anion Exchange Chromatography
Jee Hoon HAN,* Han Nim CHOI,* Sejin PARK,** Taek Dong CHUNG,*** and Won-Yong LEE*
*Department of Chemistry, Yonsei University, Seoul 120-749, Korea
**Basic Science Research Institute, Sungshin Women’s University, 249-1 Dongsun-dong, Seongbuk-gu, Seoul 136-742, Korea
***Department of Chemistry, Seoul National University, Seoul 151-747, Korea
A simple amperometric detection (AD) method based on mesoporous platinum (Pt) electrodes has been developed for the determination of sugars (glucose, fructose, and sucrose) with high-performance anion-exchange chromatography (HPAEC). The amperometric detection is based on the direct oxidation of sugars on mesoporous Pt films formed on a gold electrode. The mesoporous Pt electrode (roughness factor of 243) sensitively responded to glucose, fructose, and sucrose in 80 mM sodium hydroxide solution as an alkaline mobile-phase for HPAEC. Under the optimum conditions, the limits of detection (S/N = 3) in these sugars were 0.24, 0.29, and 1.8 mM, for glucose, fructose, and sucrose, respectively. The reproducibility (relative standard deviation) of the measurements was less than 3.5%. The present method was applied to the determination of sugars in apple juice. The recoveries for all sugars ranged from 97 to 99%.