Abstract − Analytical Sciences, 26(7), 767 (2010).
Luminol Encapsulated Liposome as a Signal Generator for the Detection of Specific Antigen-antibody Reactions and Nucleotide Hybridization
Pakavadee RAKTHONG,* Akarin INTARAMAT,** and Kavi RATANABANANGKOON*,**
*Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
**Chulabhorn Research Institute and Chulabhorn Graduate Institute, Bangkok 10210, Thailand
**Chulabhorn Research Institute and Chulabhorn Graduate Institute, Bangkok 10210, Thailand
Liposomes prepared with biotinylated phospholipids and luminol entrapped were shown to be of 187 nm in size, 59% of which were unilamellar and with 43% luminol trapping efficiency. Liposome prepared from biotinylated phospholipids with a longer hydrophilic PEG2000 spacer, but not with the shorter hydrophobic caproyl one, bound efficiently and specifically with immobilized streptavidin in a microplate assay. The interactions of dinitrophenol and tobramycin with their respective antibodies, and the hybridization of 20-mers oligonucleotides were studied using the liposome as a signal generator. These reactions were shown to be specific with limits of detection of 0.58 μM, 0.96 μM and 18 nM, respectively.
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