Analytical Sciences

Abstract − Analytical Sciences, 26(1), 51 (2010).

Determination of Acid Dissociation Constants of Compounds Active at Neuronal Nicotinic Acetylcholine Receptors by Means of Electrophoretic and Potentiometric Techniques
Gabriella RODA, Clelia DALLANOCE, Giovanni GRAZIOSO, Vincenzo LIBERTI, and Marco De AMICI
Dipartimento di Scienze Farmaceutiche "Pietro Pratesi", Università degli Studi di Milano, Via Mangiagalli 25, 20133 Milano, Italy
The dissociation constants of epiboxidine 2 and a series of bases active at neuronal nicotinic acetylcholine receptors were determined by means of potentiometric and electrophoretic methods, which gave values in good agreement. Although showing different features, the two techniques are complementary for dissociation constant determinations. The choice of the most suitable method is guided by the available amount of sample, its purity, and the time needed for the analysis. The experimental values were compared with the predictions obtained with ACD/pKa DB software.