Analytical Sciences

Abstract − Analytical Sciences, 26(1), 121 (2010).

Quantitative Spot-Test Analysis of Metformin in Pharmaceutical Preparations Using Ultraviolet-Visible Diffuse Reflectance Spectroscopy
Matthieu TUBINO,* Luís Francisco BIANCHESSI,* and Marta M. D. C. VILA**
*Institute of Chemistry, University of Campinas, P. O. Box 6154, BR-13083-970 Campinas, SP, Brazil
**University of Sorocaba, Sorocaba, SP, Brazil
A quantitative spot-test for the determination of metformin in pharmaceutical preparations using diffuse UV-visible reflectance is reported. The procedure is quite simple, involving in the formation of a metformin-nickel(II) complex on a glass filter membrane with a later measurement of the reflectance in the spectrophotometer using an integration sphere. The analytical results obtained with commercial products were statistically compared with those resulting from a method recommended by JP and by USP, where complete agreement was observed. The average RSD is 2.5% and the detection (0.009 mol L−1) and the quantitation (0.03 mol L−1) limits are quite adequate for pharmaceutical analysis.