Analytical Sciences

Abstract − Analytical Sciences, 25(9), 1159 (2009).

Immobilization of Glucose Oxidase on a Poly(dimethylsiloxane) Layer by Using Poly(L-lysine) as a Polymer Backbone
Tomoyuki YASUKAWA,*,** Eiji MAEKAWA,* and Fumio MIZUTANI*
*Graduate School of Material Science, University of Hyogo, 3-2-1 Kouto, Kamigori, Ako, Hyogo 678-1297, Japan
**JST-CREST, 3-2-1 Kouto, Kamigori, Ako, Hyogo 678-1297, Japan
Poly(dimethylsiloxane) (PDMS)-coated platinum electrodes modified with glucose oxidase (GOx) have been prepared from poly(L-lysine) (polymer backbone), glutaraldehyde (cross-linking agent) and poly(ethylene glycol) units. To fabricate a GOx layer by applying cross-linking chemistries, the PDMS layer was treated with oxygen plasma to replace silane groups with silanol groups. Optimization for the chemical fabrication of a GOx layer resulted in a simple preparation of sensors with a wide detectable range (0.1 − 6.0 mM) and without interference from hydrogen peroxide produced by a GOx reaction and the other in biological samples.