Analytical Sciences

Application of Adsorptive Voltammetry for the Detection of Sub-nano Molar Cyclizine in Biological Fluids and Tablets Using Fast Fourier Transform Continuous Cyclic Voltammetry in a Flowing System

Parviz NOROUZI,*¹ Rassoul DINARVAND,*² Mohammad Reza GANJALI,*¹ Aliakbar MOOSAVI-MOVAHEDI,*³ Aliakbar SABOURY,*³ and Atefeh TAMADDON*⁴

*1 Center of Excellence in Electrochemistry, Department of Chemistry, University of Tehran, Tehran, Iran
*2 Medical Nanotechnologycal Research Center, University of Tehran/Medical Sciences, Tehran, Iran
*3 Institute of Biochemistry & Biophysics, University of Tehran, Tehran, Iran
*4 Department of Chemistry, Central Tehran Unit, Islamic Azad University, Tehran, Iran

An easy and fast Fourier transform continuous cyclic voltammetric technique (FFTCV) for monitoring of ultra trace amounts of cyclizine in a flow-injection system has been introduced in this work. The potential waveform, which was applied continuously on an Au disk microelectrode (12.5 µm in radius) consisted of the potential steps for cleaning, accumulation and potential ramp. The proposed detection method has some advantages, the greatest of which are as follows: first, it is no longer necessary to remove oxygen from the analyte solution and second, this is a very fast and appropriate technique for determination of the drug compound in a wide variety of chromatographic analysis methods. The detection limit for cyclizine was 1.8 ng ml^-1. The relative standard deviation (RSD) of the proposed technique at 5.0 × 10^-7 was 2.0 for 10 runs. The influences of pH of eluent, accumulation potential, sweep rate, and accumulation time on the determination of the cyclizine were considered. The proposed method was applied to the determination of cyclizine in a pharmaceutical preparation.

J-STAGE: View this article in J-STAGE