Analytical Sciences

Abstract − Analytical Sciences, 25(3), 359 (2009).

DNA-Binding and Cleavage Studies of a Novel Two-dimensional Manganese(II) Azide Complex with N-Methylimidazole
Fengjuan CHEN, Zhihong XU, Pinxian XI, Xiaohui LIU, and Zhengzhi ZENG
State Key Laboratory of Applied Organic Chemistry, and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China
A new complex, manganese(II) azide complex with N-methylimidazole, has been synthesized and characterized by elemental analysis, IR spectra and single crystal X-ray studies. Absorption spectroscopy, emission spectroscopy, viscosity measurements and CD spectroscopy have been used to investigate the binding of the complex with calf thymus DNA (CTDNA). The intrinsic binding constant Kb of the complex with DNA was obtained by electronic absorption titration; the value is consistent with the result by fluorescence titration method. The spectroscopic studies together with viscosity measurements support the claim that the title complex bonds to CT-DNA by a groove mode. Control cleavage experiments using pBR 322 plasmid DNA which suggest minor groove binding for the complex.