Analytical Sciences

Abstract − Analytical Sciences, 25(12), 1381 (2009).

Rolling Circle Amplification for Signal Enhancement in Ovalbumin Detection
Toshiro KOBORI, Atsuko MATSUMOTO, Hirokazu TAKAHASHI, and Shigeru SUGIYAMA
Nanobiotechnology Laboratory, National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kan-nondai, Tsukuba 305-9642, Japan
We have developed a rolling circle amplification-procedure as a method to enhance signals of immunoassay (immuno-RCA) to detect one of the major food allergens, ovalbumin (OVA). Immuno-RCA in combination with a fluorescent dye and a circular DNA probe prepared by intramolecular ligation easily allowed the real-time detection of OVA, and could apparently detect signals from OVA with concentrations ranging from 10−12 to 10−7 g/mL. Therefore, sensitive and easily handled aspects of this method would contribute to an effective signal enhancement in immunoassay for food allergen detections.