Analytical Sciences

Abstract − Analytical Sciences, 25(11), 1365 (2009).

Electrochemical Study of Functionalization on the Surface of a Chitin/Platinum-modified Glassy Carbon Paste Electrode
Kazuharu SUGAWARA,*1 Asako YUGAMI,*2 Norifumi TERUI,*3 and Hideki KURAMITZ*4
*1 Faculty of Engineering, Maebashi Institute of Technology, Maebashi, Gunma 371-0816, Japan
*2 Faculty of Education, Gunma University, Maebashi, Gunma 371-8510, Japan
*3 Department of Chemical Engineering, Ichinoseki National College of Technology, Ichinoseki, Iwate 021-8511, Japan
*4 Department of Environmental Biology and Chemistry, Faculty of Science, University of Toyama, Toyama 930-8555, Japan
To functionalize chitin surfaces using proteins, we developed a glucose oxidase (GOD)-chitin/platinum-modified glassy carbon paste electrode (GCPE) as a model. In a weakly acidic solution, negatively charged GOD were immobilized by the protonated acetylamide groups on chitin. When the electrode was immersed in a solution containing GOD, the enzyme was readily immobilized due to the electrostaic interaction. In addition, measurements were performed using electrodes made with powders of different sizes because sensor performance depends on the particle sizes of glassy carbon powder.