Analytical Sciences

Abstract − Analytical Sciences, 25(1), 125 (2009).

Spectrophotometric Determination of Quinolone Antibiotics by an Association Complex Formation with Aluminum(III) and Erythrosin
Takako YAMAGUCHI, Masahiro NAKAO, Ryosuke NAKAHARA, Yuka NISHIOKA, Chiho IKEDA, and Yoshikazu FUJITA
Osaka University of Pharmaceutical Sciences, Nasahara, Takatsuki, Osaka 569-1094, Japan
A simple and sensitive spectrophotometric method for the determination of quinolone antibiotics was established based on an association complex formation with aluminum(III) and erythrosin. In the determination of ofloxacin as a quinolone antibiotic, Beer's law is obeyed in the range of 0.1 - 3.2 µg ml-1, with an effective molar absorptivity at 555 nm and the relative standard deviation being 1.2 × 105 L mol-1 cm-1 and 0.9% (n = 6). This method was successfully applied to the assay of quinolone antibiotics in pharmaceutical preparations.