Analytical Sciences

Abstract − Analytical Sciences, 24(9), 1117 (2008).

Distribution and Dynamic Pathway of Selenium Species in Selenium-deficient Mice Injected with 82Se-enriched Selenite
Kaori SHIGETA,* Kentaro MATSUMURA,* Yoshinari SUZUKI,* Atsuko SHINOHARA,** and Naoki FURUTA*
*Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo, Tokyo 112-8551, Japan
**Department of Epidemiology and Environmental Health, Department of Internal Medicine, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo, Tokyo 113-0033, Japan
In order to elucidate Se metabolism in a living body, 82Se-enriched selenite was injected intravenously into mice fed Se-adequate and -deficient diets. We studied the time-dependent changes in the distribution of the labeled Se in organs, red blood cells, and plasma. The total Se was determined by flow-injection ICPMS, and Se speciation analysis was conducted by micro-affinity chromatography coupled with low-flow ICPMS. Total Se in almost all organs, including liver, showed the maximum at 1 h after injection. From speciation analysis, exogenous 82Se as Se-containing proteins other than selenoprotein P (Sel-P) (selenium containing albumin (SeAlb) and extra cellular glutathione peroxidase (eGPx)), peaked at 1 h and quickly decreased from 1 to 6 h after injection, whereas that as Sel-P, peaked at 6 h, and gradually decreased from 6 to 72 h after injection. We found that there were two pathways for the transfer of Se in mice; one was as SeAlb until 1 h after injection, and the other was as Sel-P from 6 to 72 h after injection.