Analytical Sciences

Abstract − Analytical Sciences, 24(7), 865 (2008).

HPLC Separation of Triacylglycerol Positional Isomers on a Polymeric ODS Column
Ikuma KURODA,* Toshiharu NAGAI,** Hoyo MIZOBE,** Nobuhito YOSHIMURA,*** Naohiro GOTOH,*** and Shun WADA***
*GL Sciences Inc., 237-2 Sayamagahara, Iruma, Saitama 358-0032, Japan
**Tsukishima Foods Industry Co., Ltd., 3-17-9 Higashi Kasai, Edogawa, Tokyo 134-8520, Japan
***Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo 108-8477, Japan
A polymeric ODS column was applied to the resolution of triacylglycerol positional isomers (TAG-PI), i.e. 1,3-dioleoyl-2-palmitoyl-glycerol (OPO) and 1,2-dioleoyl-3-palmitoyl-rac-glycerol (OOP), with a recycle HPLC system. To investigate the ODS column species and the column temperatures for the resolution of a TAG-PI pair, a mixture of OPO and OOP was subjected to an HPLC system equipped with a non-endcapped polymeric, endcapped monomeric, endcapped intermediate, or non-endcapped monomeric ODS column at three different column temperatures (40, 25, or 10°C). Only the non-endcapped polymeric ODS column achieved the separation of OPO and OOP, and the lowest column temperature (10°C) showed the best resolution for them. The other pair of TAG-PI, a mixture of 1,3-dipalmitoyl-2-oleoyl-glycerol (POP) and 1,2-dipalmitoyl-3-oleoyl-rac-glycerol (PPO) was also subjected to the system equipped with a non-endcapped polymeric or monomeric ODS column at five different column temperatures (40, 32, 25, 17, and 10°C). Thus, POP and PPO were also separated on only the non-endcapped polymeric ODS column at 25°C. However, no clear peak appeared at 10°C. These results would indicate that the polymeric ODS stationary phase has an ability to recognize the structural differences between TAG-PI pairs. Also, the column temperature is a very important factor for separating the TAG-PI pair, and the optimal temperature would relate to the solubility of TAG-PI in the mobile phase. Furthermore, the recycle HPLC system provided measurements for the separation and analysis of TAG-PI pairs.