Analytical Sciences


Abstract − Analytical Sciences, 24(2), 297 (2008).

Microscopic Measurement of Circular Dichroism Spectra
Aira MATSUGAKI, Hideaki TAKECHI, Hideaki MONJUSHIRO, and Hitoshi WATARAI
Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan
A microscope device to measure the circular dichroism (CD) spectra of a specified microscopic region of chiral samples was constructed by combining of a couple of objective lenses and a CCD camera, which was installed in a sample chamber of a commercially available CD spectropolarimeter. By using this apparatus, high quality micro-CD spectra in the 60 × 60 µm region of samples could be measured. Micro-CD spectra of thin film of chiral DNA samples on glass and a natural kidney bean leaf were measured, and the potential of the micro-CD apparatus was successfully demonstrated.