Analytical Sciences

Abstract − Analytical Sciences, 24(12), 1619 (2008).

Detection of an Antibody to Avian Influenza Virus by an Electrochemical Immunoassay (eELISA)
Keiichi OHTSUKA,* Hiroshi ENDO,** Kimio MORIMOTO,** Bui N. VUONG,*** Haruko OGAWA,*** Kunitoshi IMAI,*** and Shigeori TAKENAKA*
*Department of Applied Chemistry, Kyushu Institute of Technology, 1-1 Sensui, Tobata, Kitakyushu 804-8550, Japan
**Hano Manufacturing Co. Ltd., 1-1-12 Hakomatsu, Higashi, Fukuoka 812-0061, Japan
***Research Center for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro 080-8555, Japan
The detection of an antibody against nucleoprotein (NP) of the avian influenza virus (AIV) was attempted by an electrochemical immunoassay by combining a secondary antibody-alkaline phosphatase conjugate and 4-aminophenylphosphate. Released 4-aminophenol was quantitated electrochemically, and the current derived from oxidation of the hydrolysis product increased linearly over a wide primary antibody concentration range (10 - 1000 ng/ml). The detection limit of this electrochemical immunoassay for the antibody against the NP of AIV was found to be 10 ng/ml.