Analytical Sciences

Abstract − Analytical Sciences, 24(12), 1525 (2008).

Live Single-cell Metabolomics of Tryptophan and Histidine Metabolites in a Rat Basophil Leukemia Cell
Hajime MIZUNO, Naohiro TSUYAMA, Sachiko DATE, Takanori HARADA, and Tsutomu MASUJIMA
Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami, Hiroshima 734-8553, Japan
A direct and rapid metabolic analysis of a live single cell was performed by live single-cell video-mass spectrometry. The contents of the cytoplasm and a granule were sucked into a nano-electrospray ionization (nano-ESI) tip, and were directly introduced into a Q-TOF mass spectrometer by nano-spray after the addition of an ionization solvent. The metabolic pathways and the locations of tryptophan and histidine metabolites were traced by this method for a cultured rat basophil leukemia cell line (RBL-2H3). The t-values of detected peaks by a t-test between the different location, e.g. cytoplasm and a granule, revealed the molecular localization of each MS peak. A direct and quick metabolomic analysis of a living cell under simultaneous video-microscopic observations was innovated.