Analytical Sciences

Abstract − Analytical Sciences, 24(1), 149 (2008).

A Novel Chip Device Based on Wired Capillary Packed with High Performance Polymer-based Monolith for HPLC: Reproducibility in Preparation Processes to Obtain Long Columns
Ken HOSOYA,* Mari SAKAMOTO,* Kunihiko AKAI,** Tomoko MORI,* Takuya KUBO,* Kunimitsu KAYA,* Kazuma OKADA,*** Norio TSUJIOKA,*** and Nobuo TANAKA***
*Graduate School of Environmental Studies, Tohoku University, Aoba 6-6-20, Aramaki Aoba, Sendai 980-8579, Japan
**Hitachi Chemical Company, 1500 Ogawa, Chikusei, Ibaraki 308-8521, Japan
***Graduate School of Science & Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo, Kyoto 606-8585, Japan
This report describes the development of novel wired chip devices for μ-HPLC analyses. The monolithic capillary column to be wired was prepared using a tri-functional epoxy monomer, tris(2,3-epoxypropyl)isocyanurate with a diamine, 4-[(4-aminocyclohexyl)methyl]cyclohexylamine. The prepared column was evaluated by SEM observation of the sectional structure of column and μ-HPLC. In addition, the reproducibility in the preparation of long capillary columns having nearly 1 m length was extensively examined for applications of novel wired chip devices. The authors demonstrated that the monolithic structure of the prepared long capillary could be finely controlled under the strictly maintained operational conditions and thus the relative standard deviation (RSD) of the column properties such as the number of theoretical plates, retention factor, and permeability could be well controlled to become less than 10%. Furthermore, the wired chip device column showed that its high performance was kept even after chip preparation.