Analytical Sciences

Abstract − Analytical Sciences, 23(5), 597 (2007).

Flow Injection Chemiluminescence Determination of Imipramine in Tablets, Human Plasma and Urine Samples Based upon Oxidation with Singlet Oxygen Generated in N-Bromosuccinimide-Hydrogen Peroxide Reaction
Liang HAO,*,** Jianxiu DU,* and Jiuru LU*
*Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, People's Republic of China
**Department of Chemistry, Shaanxi University of Technology, Hanzhong 723001, People's Republic of China
Singlet oxygen generated in a reaction between N-bromosuccinimide and hydrogen peroxide was used for the chemiluminescence oxidation of imipramine. A strong chemiluminescence signal was observed when imipramine was mixed with N-bromosuccinimide and hydrogen peroxide under an alkaline condition. The chemiluminescence signal was linearly dependent on the concentration of imipramine in the range of 0.01 - 1.0 mg/L. The detection limit was 0.005 mg/L imipramine and the relative standard deviation was 1.5% for a 0.4 mg/L imipramine solution in 11 repeated measurements. The proposed method was successfully applied to the determination of imipramine in tablets, human plasma and urine samples.