Analytical Sciences

Abstract − Analytical Sciences, 23(12), 1391 (2007).

Fast Separation and Quantification of C60 and C70 Fullerenes Using Thermostated Micro Thin-layer Chromatography
Pawel K. ZARZYCKI,* Hatsuichi OHTA,** Filip B. HARASIMIUK,* and Kiyokatsu JINNO**
*Section of Toxicology and Bioanalytics, Department of Environmental Biology, Koszalin University of Technology, Sniadeckich 2, 75-453 Koszalin, Poland
**Toyohashi University of Technology, School of Materials Science, Toyohashi 441-8580, Japan
Thermostated micro planar chromatography was applied for systematic separation studies of C60 and C70 fullerenes using n-alkanes as mobile phases on TLC and HPTLC plates coated with polyamide, silica gel, aluminum oxide as well as two types of octadecylsilica (C18) sorbents. Retention data were collected at constant temperature at 20°C (±0.05°C) using an unsaturated chamber mode with an eluent, such as n-pentane, n-hexane and n-heptane. The separation results under both saturated and unsaturated chamber modes for selected mobile/stationary phases were also examined, and several parameters, including separation factor (α) and resolution (RS), were compared with data obtained with high-performance liquid chromatography conditions. Interestingly, C60/C70 fullerenes separation performed on HPTLC plates with a developing distance of 45 mm was better for those observed on a 25 cm length analytical HPLC column under similar conditions to that on carbon coverage of the stationary phase, n-hexane as the mobile phase and separation temperature (RS = 1.84 and 1.68 for HPTLC, and HPLC, respectively). Moreover the advantage of the planar chromatographic separation of fullerenes studied is a short elution time of less than 6 min. Furthermore, the reported separation protocol shows a capability for the evaluation of fullerenes quantity in commercial samples.