Analytical Sciences

Abstract − Analytical Sciences, 23(1), 97 (2007).

Galactose Oxidase Immobilized on Silica in an Analytical Determination of Galactose-containing Carbohydrates
Lyudmila KONDAKOVA,* Victor YANISHPOLSKII,* Valentin TERTYKH,* and Tat'yana BUGLOVA**
*Institute of Surface Chemistry of National Academy of Sciences of Ukraine, Gen. Naumov Str. 17, 03164 Kyiv-164, Ukraine
**D.K. Zabolotny Institute of Microbiology and Vyrology of National Academy of Sciences of Ukraine, Acad. Zabolotny Str. 154, D03680 Kyiv, Ukraine
Galactose oxidase from Fusarium graminearum IMV-1060 adsorbed on, and covalently bound to, silica carriers has been used for analytical determinations of D-galactose and galactose-containing sugars. Using a flowing oxygen electrode of the Clark-type, sensor system for enzymatic analysis of water solutions of galactose-containing carbohydrates was made. Measurements were taken both in the pulse and continuous modes of a substrate flowing through a column with an immobilized biocatalyst. The linear measurement ranges for galactose-containing carbohydrates concentrations were determined.