Analytical Sciences

Abstract − Analytical Sciences, 23(1), 59 (2007).

Characterization of a Glucose Sensor Prepared by Electropolymerization of Pyrroles Containing a Tris-bipyridine Osmium Complex
Masaki TSUJIMOTO,* Tomoki YABUTANI,* Atsushi SANO,* Yuji TANI,* Hiroki MUROTANI,* Yuji MISHIMA,** Kenichi MARUYAMA,*** Mikito YASUZAWA,* and Junko MOTONAKA*
*Department of Chemical Science and Technology, Faculty of Engineering, The University of Tokushima, Minamijosanjima 2-1, Tokushima 770-8506, Japan
**Takasago Technical Center, Tsurui Chemical Co. Ltd., 2-1-1 Niihama Araicho, Takasago, Hyogo 676-0008, Japan
***Departments of Applied Chemistry and of Information and Computer Science, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku, Yokohama, Kanagawa 223-8522, Japan
A glucose sensor was developed by electrocopolymerization using pyrroles containing a tris-bipyridine (bpy) osmium complex (Os-py), pyrrole (py), pyrrole propanoic acid (PPA) and glucose oxidase (GOx) to improve the key performance characteristics, such as the sensitivity, selectivity, and long-term stability. Tris-bipyridine osmium pyrrole complexes with four different methylene moieties were utilized to correlate the methylene length with the glucose sensor performance. The electrocatalytic response of glucose was clearly observed at electrodes modified with Os-py, except for the electrode immobilized with the Os-py complex containing the shortest methylene moiety. The current response to glucose increased up to a concentration of 100 mmol dm-3. The electrocatalytic response to glucose at the [Os(bpy)2(py(6)-bpy)]2+/3+/py/PPA/GOx electrode was stable for more than 100 days. Dissolved oxygen and potential interference compounds (ascorbic acid, uric acid, and acetaminophen) minimally perturbed the current response to glucose at the [Os(DM-bpy)2(py(6)-bpy)]2+/3+/py/PPA/GOx electrode. Based on these results, a longer methylene moiety appears to improve the performance characteristics of a glucose sensor fabricated via the electropolymerization of tris-bipyridine osmium pyrrole complexes.