Analytical Sciences

Abstract − Analytical Sciences, 23(1), 17 (2007).

Colorimetric Biosensors Based on DNA-nanoparticle Conjugates
Kae SATO, Kazuo HOSOKAWA, and Mizuo MAEDA
Bioengineering Laboratory, RIKEN, Hirosawa 2-1, Wako, Saitama 351-0198, Japan
In this review, we present an overview of the technologies in colorimetric biosensors based on DNA-nanoparticle conjugates. Two types of DNA-nanoparticles aggregation assays are summarized. One of the methods relies on cross-linking of the gold nanoparticle (GNP) by hybridization. The crosslinking system was used not only to detect target DNA sequences, but also to detect metal ions or small molecules which were recognized by DNAzymes. The other method is the GNP non-crosslinking system. This approach shows high performance in the detection of single nucleotide polymorphisms. These methods do not need special equipment and open up a new possibility of point-of-care diagnoses.