Analytical Sciences

Abstract − Analytical Sciences, 22(9), 1257 (2006).

Liquid-Chromatographic Separation and Determination of Process-related Impurities, Including a Regio-Specific Isomer of Celecoxib on Reversed-Phase C18 Column Dynamically Coated with Hexamethyldisilazane
R. Nageswara RAO,* S. MEENA,*** D. NAGARAJU,* A. Raghuram RAO,*** and S. RAVIKANTH**
*HPLC/UV Group, Division of Analytical Chemistry, Discovery Laboratory, Indian Institute of Chemical Technology, Hyderabad-500007, India
**Fluoro Organic Division, Indian Institute of Chemical Technology, Hyderabad-500007, India
***Medicinal Chemistry Research Division, University College of Pharmaceutical Sience, Kakatiya University, Warangal-500009, India
A simple and rapid reversed-phase high-performance liquid-chromatographic method for the separation and determination of process-related impurities of celecoxib (CXB) in bulk drugs and pharmaceuticals was developed. The separation of impurities viz., 4-methylacetophenone (I), 1-(4-methylphenyl)-4,4,4-trifluorobutane-1,3-dione (II), 4-hydrazinobenzene sulfonamide (III) and a regio-specific isomer [3-(4-methylphenyl)-5-trifluoromethyl-1H-pyrazole-1-yl]-benzenesulfonamide (IV), was accomplished on an Inertsil ODS-3 column dynamically coated with 0.1% hexamethyldisilazane (HMDS) in acetonitrile:water (55:45 v/v) as a mobile phase and detection at 242 nm using PDA at ambient temperature. The chromatographic conditions were optimized by studying the effects of HMDS, an organic modifier, time of silanization and column temperature. The method was validated and found to be suitable not only for monitoring the synthetic reactions, but also to evaluate the quality of CXB.