Analytical Sciences

Abstract − Analytical Sciences, 22(8), 1145 (2006).

Flow-Injection Chemiluminescence Determination of Ofloxacin and Levofloxacin in Pharmaceutical Preparations and Biological Fluids
Hanwen SUN, Liqing LI, and Xueyan CHEN
College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding, 071002, China
A novel, rapid and sensitive analytical method is described for determination of ofloxacin and levofloxacin by enhanced chemiluminescence (CL) with flow-injection sampling. The method is based on the CL reaction of the Ce(IV)-Na2S2O4-ofloxacin/levofloxacin-H2SO4 system. The enhanced CL mechanism was developed and the optimum conditions for CL emission were investigated. The CL intensity was correlated linearly (r = 0.9988) with the concentration of ofloxacin (or levofloxacin) in the range of 1.0 × 10-8 - 1.0 × 10-7 g ml-1 and 1.0 × 10-7 - 6.0 × 10-6 g ml-1. The detection limit (S/N = 3) is 7 × 10-9 g ml-1. The relative standard derivation (RSD, n = 11) is 2.0% for ofloxacin at 4 × 10-7 g ml-1 and for levofloxacin at 6 × 10-7 g ml-1. This method has been successfully applied for the determination of ofloxacin and levofloxacin in pharmaceutical preparations and biological fluids with satisfactory results.