Analytical Sciences

Abstract − Analytical Sciences, 22(1), 165 (2006).

Flow Injection Spectrophotometric Determination of Bromoxynil Herbicide by Diazotization Method
M. Rasul JAN, Jasmin SHAH, and Nadia BASHIR
Department of Chemistry, University of Peshawar, N. W. F. P., Pakistan
A flow injection spectrophotometric method is proposed for the determination of bromoxynil herbicide. Bromoxynil was hydrolyzed with HCl and the resulting product, 3,5-dibromo-4-hydroxyaniline, was diazotized with nitrite and coupled with aniline. The absorbance of the azo dye was measured at 500 nm. The conditions were optimized for diazotization using FIA. The range of linearity was found to be 0.01 to 5 ppm with a molar absorptivity of 1.27 × 105 L mol-1 cm-1. The % recovery for the determination of bromoxynil was found to be 91%. The sampling frequency was 80 samples per hour for FIA. The method is simple, fast, and has been successfully applied to the determination of bromoxynil in commercial formulations and food samples.