Analytical Sciences

Abstract − Analytical Sciences, 21(5), 583 (2005).

Simultaneous Light Emitting Diode-Induced Fluorescence and Contactless Conductivity Detection for Capillary Electrophoresis
Feng TAN, Bingcheng YANG, and Yafeng GUAN
Department of Micro-Instrument and Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China
A combined detection system of simultaneous contactless conductometric and fluorescent detection for capillary electrophoresis (CE) has been designed and evaluated. The two processes share a common detection cell. A blue light-emitting diode (LED) was used as the excitation source and an optical fiber was used to collect the emitting fluorescence for fluorescent detection (FD). Inorganic ions, fluorescein isothiocyanate (FITC)-labeled amino acids and small molecule peptides were separated and detected by the combined detector, and the detection limits (LODs) of sub-µM level were achieved.