Analytical Sciences

Abstract − Analytical Sciences, 21(5), 537 (2005).

Simultaneous Determination of Carvedilol and Ampicillin Sodium by First-Derivative Fluorometry in the Presence of Human Serum Albumin
Huai You WANG, Yan XIAO, and Juan HAN
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, People’s Republic of China
A sensitive and selective method for the simultaneous determination of carvedilol and ampicillin sodium (AS) in the presence of human serum albumin (HSA) is described. The maximum emission wavelengths of carvedilol and AS are at 357 nm and 426 nm with excitation at 254 nm, respectively. The first-derivative peaks of carvedilol and AS were at 337 nm and 398 nm, respectively. The linear-regression equations of the calibration graphs of carvedilol and AS were C = 0.0001H - 0.0063 and C = 1.530H - 43.84; the correlation coefficients were 0.9990 and 0.9986, respectively. The detection limits were 1 ng ml-1 for carvedilol and 23 µg ml-1 for AS, respectively. The effects of the pH, the stability of carvedilol and AS and foreign ions on the determination of carvedilol and AS were examined. The recoveries of carvedilol and AS were measured. This method is simple and can be used for the determination of carvedilol and AS in human serum and urine samples with satisfactory results.