Analytical Sciences

Abstract − Analytical Sciences, 21(1), 57 (2005).

Rapid Separation of Microorganisms by Quartz Microchip Capillary Electrophoresis
Tomoyoshi SHINTANI,* Masaki TORIMURA,** Hiroaki SATO,** Hiroaki TAO,** and Takashi MANABE***
*Textile Industrial Institute of Ehime Prefecture, 2-5-48 Higashimura-minami Imabari, Ehime 799-1507, Japan
**Measurement Technology Research Group, Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology, 16-1 Onogawa, Tsukuba, Ibaraki 305-8569, Japan
***Department of Chemistry, Faculty of Science, Ehime University, 2-5 Bunkyo-cho, Matsuyama, Ehime 790-8577, Japan
We developed and optimized a system coupling microchip capillary electrophoresis (MCE) and laser-induced fluorescence (LIF) detection for the analysis of microorganisms. The MCE-LIF system successfully separated pure cultures of lactic acid bacteria and Saccharomyces cerevisiae within 200 s. The results indicate that the MCE system can be conveniently used for the rapid and highly sensitive detection of microorganisms. Thus, MCE can provide a cheap and simple method for the on-line detection of microbial contamination.