Analytical Sciences

Adsorption and Desorption Behaviors of Cationic Liposome-DNA Complexes upon Lipofection in Inside and Outside Biomembrane Models Using a Dynamic Quasi-Elastic Laser Scattering Method

Yoshiko UCHIYAMA,*^,** Hiroharu YUI,*^,*** and Tsuguo SAWADA*^,***

*Department of Advanced Materials Science, Graduate School of Frontier Sciences, The University of Tokyo, Kibantou 603, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8561, Japan
**Japan Society for the Promotion of Science (JSPS)
***Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Nanoarchitectonics Research Center (NARC), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan

The dynamic behaviors of cationic liposome-DNA complexes in inside and outside biomembrane models upon lipofection were investigated using the time-resolved quasi-elastic laser scattering (QELS) method. Inside and outside biomembrane models with similar phospholipid compositions to those in living cells were formed at a tetradecane/phosphate buffered saline (TD/PBS) interface. Cationic liposome-DNA complexes were injected into the buffer subphase, and their adsorption/desorption behaviors at the biomembrane models were monitored through changes in the interfacial tension. We found that the adsorption rate of the complexes increased 2.6 times more in the outside model than in the inside one. The adsorption rate of DNA alone did not show a remarkable difference from one side to the other; however, the adsorption rate of the cationic liposome alone showed a similar tendency to that of the liposome-DNA complex. These results indicated that the difference in lipid composition induced a different dynamic behavior of exogenous biomolecules and that the cationic liposomes played an important role in the faster incorporation of DNA into cells upon lipofection.

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