Analytical Sciences

Abstract − Analytical Sciences, 20(1), 129 (2004).

Determination of Hydroxylated Polycyclic Aromatic Hydrocarbons in Airborne Particulates by High-Performance Liquid Chromatography with Fluorescence Detection
Naoya KISHIKAWA, Seigo MORITA, Mitsuhiro WADA, Yoshihito OHBA, Kenichiro NAKASHIMA, and Naotaka KURODA 
Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan
This paper describes a highly sensitive and selective method for the determination of hydroxylated polycyclic aromatic hydrocarbons (PAHs-OH) in airborne particulates. PAHs-OH were extracted from airborne particulates with ethanol by ultrasonication. The extractant was further cleaned up by solid phase extraction (SPE) with an aminopropylsilica cartridge, and subsequently analyzed by reversed-phase high-performance liquid chromatography with fluorescence detection. 2-Hydroxy-1-acetonaphthone was used as an internal standard. By the proposed method, 2-hydroxyfluorene and 1-hydroxypyrene were identified in airborne particulates and their concentrations were determined for the first time.