Analytical Sciences

Optimization of HPLC-ECD Conditions for Determination of Catechins with Precision and Efficiency Based on the FUMI Theory

Akira KOTANI,* Yuzuru HAYASHI,** Rieko MATSUDA,** and Fumiyo KUSU* 

*School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1, Horinouchi, Hachioji, Tokyo 192-0392, Japan
**National Institute of Health Sciences, 1-18-1, Kami-Yoga, Setagaya, Tokyo 158-8501, Japan

A chemometric tool based on the Function of Mutual Information (FUMI) theory can provide a relative standard deviation (RSD) without repetitive measurements in high-performance liquid chromatography with electrochemical detection (HPLC-ECD). Two parameters: precision (= information content) φ and efficiency (= information content/time) θ, which were calculated from predicted RSD based on the FUMI theory, were used to optimize HPLCECD conditions, such as applied potential, flow rate, column length, and size of ODS porous packing. We selected catechins as analytes, and found that the most optimum applied potential and flow rate were +600 mV vs. SCE and 0.9 mL/min, respectively, because they gave the largest φ and θ values. Buffer concentration in mobile phase is less effective for giving large φ and θ values. Since the FUMI theory makes it possible to predict RSD without repetitive measurements, the present method saves considerable amounts of chemicals and experimental time, and was found to be useful for the optimization of experimental conditions for determination by HPLC-ECD.

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