Analytical Sciences

Direct Determination of Benzamides in Serum by Column-Switching High-Performance Liquid Chromatography

Ryoko CHIBA,*  Ayako OGASAWARA,* Teppei KUBO,* Hiroyuki YAMAZAKI,** Masuo UMINO,** and Yoichi ISHIZUKA***

*Showa Pharmaceutical University, Machida 194-8543, Japan
**Scientific Instrument Division, Tosoh Co., 2743-1, Hayakawa, Ayase 252-1123, Japan
***Teikyo University School of Medicine, Mizonokuchi Hospital 3-8-3, Kawasaki 213-0001, Japan

A column-switching high-performance liquid chromatographic method with fluorescence detection was developed for the simultaneous determination of four benzamide-type anti-psychotic drugs: sulpiride, tiapride, sultopride and metoclopramide in human serum. In this method, a TSKgel Super-ODS column was used as an analytical column, and a TSKgel G 2000SW was prepared as a pretreatment column. Under the optimized analytical conditions, four benzamidetype anti-psychotic drugs were eluted within 18 min. The detection limits (S/N = 3) for sulpiride, tiapride, sultopride and metoclopramide are 1 ng/ml, 4 ng/ml, 2 ng/ml and 0.5 ng/ml, respectively. Finally, the method was applied to the determination of sulpiride in human serum samples obtained after a single oral dose of sulpiride.

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