Analytical Sciences

Abstract − Analytical Sciences, 19(3), 437 (2003).

Quartz-Crystal Microbalance Immunosensor for Schistsoma-Japonicum-Infected Rabbit Serum
Zhao-Yang WU,*  Guo-Li SHEN,*  Shi-Ping WANG,** and Ru-Qin YU*
*State Key Laboratory of Chemo/Biosensing and Chemometrics, Institute of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
**Department of Parasitology, Central South University, Changsha, 410081, China
A quartz-crystal microbalance immunosensor (QCM) has been developed for the direct determination of Schistosoma-japonicum-infected rabbit serum. A self-assembled monolayer with carboxyl groups was first coated on a gold electrode of a quartz-crystal resonator by the spontaneous adsorption of 3-mercaptopropionic acid. Schistosoma-japonicum molecular antigen of 32 kD molecular weight was then covalently attached to the crystal surface. The QCM immunosensor was used to detect infected rabbit serum (IRS49-2000); a maximum titer of 1:800 was achieved.