Analytical Sciences

Abstract − Analytical Sciences, 19(1), 175 (2003).

Range of Separation of Potential Tool for Bioseparation, Microchip Electrophoresis System, for DNA Polymorphisms on the Human Y-Chromosome
Mohammad JABASINI,* Feng XU,*1,*3 Fuquan DANG,*1 Toshikatsu SHINKA,*2 Yutaka NAKAHORI,*2 and Yoshinobu BABA*1,*4
*1 Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima, CREST, Japan Science and Technology Corporation (JST), Tokushima 770-8505, Japan
*2 Department of Human Genetics Public Health, School of Medicine, The University of Tokushima, Tokushima 770-8505, Japan
*3 Life Science Department, Shimadzu Corporation, 1 Nishinokyo-Kuwabara-cho, Kyoto 604-8511, Japan
*4 Single-Molecule Bioanalysis Laboratory, National Institute of Advanced Industrial Science and Technology (AIST), Hayashi-cho 2217-14, Takamatsu 761-0395, Japan
For the requirement of a high, fast and sufficient technology to suit the needs of 21st century biotechnology, the separation range of a microchip electrophoresis system was studied. Two DNA fragments on the human Y-chromosome, SY594 (82 bp) and 12f2 (88 bp), were successfully separated with a reproducibility of 1.9% and an accuracy of 2.8%. Then, a mixture of 10 DNA markers ranging from 61 bp to 189 bp was successfully separated with high resolution. All of these results demonstrate the superiority of microchip electrophoresis as a tool for 21st century bioseparation.