Analytical Sciences

Abstract − Analytical Sciences, 19(1), 171 (2003).

Novel Observation of a Circular Dichroism Band Originating from Amyloid Fibril
Hiroshi ABE*,** and Hiroshi NAKANISHI*,** 
*Biological Information Research Center, National Institute of Advanced Industrial Science and Technology, 1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan
**Department of Biological Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-0022, Japan
A peptide fragment of a non-amyloid-beta omponent (NAC(1-13)) was studied by CD and electron microscopy. Typical amyloid fibrils were observed by EM in a solution of NAC(1-13). In addition to the beta-structural CD band in the far-UV region, a novel CD band near 285 nm was observed in a peptide solution of NAC(1-13). Taking the NAC(1-13) to contain neither an aromatic amino acid residue nor cystine into account, the CD band can be attributed to amyloid fibrils of NAC(1-13).