Abstract − Analytical Sciences, 18(4), 409 (2002).
Investigation of the Chemico-physicalCharacteristics of the Active Components in the Chinese Herb Gastrodiaelata Bl. by Capillary Zone Electrophoresis
DexianWANG,*,*** GengliangYANG,*,** Baohui LI,*Zhiwei LI,* and Yi CHEN**
*Department of Chemistry, HebeiUniversity, Baoding 071002, P. R. China
**Center for Molecular Science,Institute of Chemistry, Chinese Academy of Science, Beijing 100080, P. R.China
***Hebei Medical College for Continue Education, Baoding 071000,P. R. China
**Center for Molecular Science,Institute of Chemistry, Chinese Academy of Science, Beijing 100080, P. R.China
***Hebei Medical College for Continue Education, Baoding 071000,P. R. China
A CE method for investigating thechemico-physical characteristics of the active components of low molecularweight in Gastrodia elata Bl. is described. First, the dissociationconstants of five active components were determined based on the relationbetween the effective mobility of the solutes and the buffer pH value.Second, an equation that describes the relation of the migration time andthe molecular weight was developed and used to predict the migration orderand to calculate the electroosmotic velocity. The results predicted bytheory agreed well with that from experiments.
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