Analytical Sciences

Abstract − Analytical Sciences, 18(1), 65 (2002).

An Attempt Directed toward Enhanced Shape Selectivity in Reversed-Phase Liquid Chromatography: Preparation of the Dodecylaminated beta-Cyclodextrin-Bonded Phase
Chuzo FUJIMOTO,*  Akira MAEKAWA,** Yumiko MURAO,** Kiyokatsu JINNO,** and Tsutomu TAKEICHI** 
*Department of Chemistry, Hamamatsu University School of Medicine, Hamamastu 431-3192, Japan
**School of Materials Science, Toyohashi University of Technology, Toyohashi 441-8580, Japan
With the aim of preparing a stationary phase with a high shape-recognition ability for liquid chromatography, a new bonded phase was synthesized by coupling multiply dodecylamino-substituted beta-cyclodextrin (beta-CD) to 3-glycidoxypropyl-derivatized silica gel. The stationary phase prepared in this way was expected to have increased shape selectivity compared with that of conventional reversed-phase materials, due to solute interactions with the alkyl chain piles built up on the beta-CDs bonded to silica. The separation characteristics of the bonded phase were investigated using polycyclic aromatic hydrocarbons (PAHs) with different molecular shapes and compared with those of monomeric ODS and native beta-cyclodextrin-bonded phases. The newly developed stationary phase was found to be highly selective for PAHs.