Analytical Sciences

Abstract − Analytical Sciences, 17(1), 167 (2001).

Derivative Matrix Isopotential Synchronous Fluorescence Spectroscopy for the Direct Determination of 1-Hydroxypyrene as a Urinary Biomarker of Exposure to Polycyclic Aromatic Hydrocarbons
Yao-Qun LI,  Wei SUI, Chun WU, and Li-Jun YU
The Key Laboratory of Analytical Sciences of MOE and Department of Chemistry, Xiamen University, Xiamen 361005, China
Urinary 1-hydroxypyrene is a biomarker in the measurement of human exposure to polycyclic aromatic hydrocarbons. A rapid and simple derivative isopotential synchronous fluorescence method was developed for the direct determination of 1-hydroxypyrene in urine. A length of iso-intensity route was scanned on the three-dimensional fluorescence spectrum of urine and this result was combined with that from derivative technique. Thus the strong background signals of urine were removed and the 1-hydroxypyrene can be determined directly in urine without tedious pre-separation. The derivative isopotential synchronous fluorescence spectrum was directly obtained from a single scan on a spectrofluorometer, which further simplified isopotential synchronous fluorescence technique. The recoveries of 93% to 115% were obtained for 1-hydroxypyrene added to urine.