Analytical Sciences

Abstract − Analytical Sciences, 16(2), 107 (2000).

Design and Application of Piezoelectric Quartz Crystal-based Immunoassay
Xiaodi SU,* ˇ€ˇÄook Tim CHEW,** and Sam F. Y. LI*
*Institute of Material Research and Engineering, 3, Research Link, Singapore 117602
**Paediatrics Department, Medical Faculty, National University of Singapore,10 Kent Ridge Crescent, Singapore 119260
This paper describes some basic test schemes of piezoelectric quartz crystal microbalance (QCM)-based immunoassay.These include one-step direct assay, one-step indirect assay, two-step sandwiched assay, displacement assay, and mass-amplifiedassay. Applications of these designs in the fields of clinic diagnosis, food analysis, environmental analysis, andveterinary diagnosis are reviewed. In addition, some critical issues of QCM immunoassay are also dealt with, includingthe evaluation of liquid phase and gas phase detection, and the biomolecules' coating techniques, non-specific adsorption,and regeneration of used crystals.