Analytical Sciences

Abstract − Analytical Sciences, 15(12), 1191 (1999).

Solvatochromic Study of the Separation of Glycyl-Leucyl-Phenylalanine and Some Homologous by Reversed-Phase High-Performance Liquid Chromatography
Sylvie RABOUAN*, Patrice PROGNON**, Danix·Æ BARTHES* +
*Laboratoire de Chimie Analytique, UFR Mdecine et Pharmacie, B.P. 199, 86005 Poitiers Cedex, France
**Laboratoire de Chimie Analytique, Facult des Sciences Pharmaceutiques et Biologiques de Paris Sud, 92290 Chatenay Malabry, France
The separation of glycyl-leucyl-phenylalanine and its homologous was studied by reversed phase high-performance liquid chromatography. A solvatochromic interpretation (the linear solvation energy relationship and ReichardtÕó E N T scale) and a non solvatochromic interpretation (Prs factor) showed that the optimum eluent for convenient separation on a C18 sta-tionary phase was acetonitrile/0.1 M (mol l -1 ) sodium citrate buffer pH 3.0, 12/88 (v/v). The retention mechanism involved appeared to be governed mainly by dipole-dipole interaction and to be influenced by the hydrogen acceptor nature of acetonitrile. In the experimental conditions, separation was only slightly influenced by the ionized status (cationic form) of the studied peptides. Finally, separation was improved by using a phenyl-bonded silica stationary phase. (Keywords: Oligopeptides, solvatochromic parameters, high-performance liquid chromatography, glycyl-leucyl-phenylalanine)